NTUBST2023EN

A. Human induced pluripotent stem cell biology and application

• Mechanism of cell reprogramming
• iPSC generation
• Differentiation of hepatic/pancreatic cells from human iPS cell
• Differentiation of endothelial cells from human iPS cell

B. Cancer stem cell (liver cancer and colon cancer)

• Self-renewal mechanism of cancer stem cells.
• Screening novel cancer stem cell surface markers by phage display system.

C. Drug delivery (nanoparticle)

• HCC and Colon cancer target-drug developing.

1. Huang CY, Lok YY, Lin CH, Lai SL, Wu YY, Hu CY, Liao CB, Ho CH, Chou YP, Hsu YH, Lo YH, Chern E*.  Highly Reliable GIGA-sized Synthetic Human Therapeutic Antibody Library Construction. Front. Immunol. 2023 Aprl; 14:1089395 (SCI, *corresponding author)​​ 
2. Chiang MC, Chern E*. Ocular surface microbiota: Ophthalmic infectious disease and probiotics. Front Microbiol. 2022 Aug; 19;13:952473. (SCI, *corresponding author)​​ 
3. Chiang MC, Chern E*. More than Antibiotics: Latest Therapeutics in the Treatment and Prevention of Ocular Surface Infections. J Clin Med. 2022 Jul; 19;11(14):4195. (SCI, *corresponding author)​​ 
4. Chiang MC, Chern E*. Current Development, Obstacle and Futural Direction of Induced Pluripotent Stem Cell and Mesenchymal Stem Cell Treatment in Degenerative Retinal Disease. Int J Mol Sci, 2022 Feb; 25;23(5):2529. (SCI, *corresponding author) 
5. Chang PH, Chao HM, Chern E*, Hsu SH*. Chitosan 3D cell culture system promotes naïve-like features of human induced pluripotent stem cells: A novel tool to sustain pluripotency and facilitate differentiation Biomaterials, 2021 Jan; 268:120575. (SCI, *corresponding author)​​ 
6. Wang TW^#, Chern E^#, Hsu CW, Tseng KC, Chao HM. SIRT1-mediated expression of CD24 and epigenetic suppression of novel tumor suppressor miR-1185-1 increases colorectal cancer stemness. Cancer Research, 2020 Dec; 1;80(23):5257-5269 (SCI, ^#co-first author) 
7. Chen YH, Lin DC, Chern E, Huang YY. The use of micro-needle arrays to deliver cells for cellular therapies. Biomed Microdevices, 2020 Sep; 5;22(4):63. (SCI)​​ 
8. Chao YC, Hong YJ, Lee CY, Zhuang SC, Wu MT, Lee YY, Lee HY, He YS, Yu HY, Huang YZ, Chern E*, Jiang HR*. NanoMuscle: Controllable Contraction and Extension of Mechanically Interlocked DNA Origami. Nanoscale, 2020,12, 2992-2998 (SCI, *corresponding author)​​ 
9. Chao HM, Chern E*. Patient-derived induced pluripotent stem cells for models of cancer and cancer stem cell research. Journal of the Formosan Medical Association 2018, 117(12):1046-1057 (SCI, *corresponding author) 
10. Chou MJ, Yu HY, Hsia RJ, Chen YH, Hung TT, Chao HM, Chern E*, Huang YY*. Highly efficient Intracellular protein delivery by cationic polyethyleneimine-modified gelatin nanoparticle. Materials 2018, 11(2), 301 (SCI, *corresponding author) 
11. Chang PH, Sekine K, Chao HM, Hsu Sh*, Chern E *. Chitosan promotes cancer progression and stem cell properties in association with Wnt signaling in colon and hepatocellular carcinoma cells. Scientific Reports. 2017; Apr 3;8:45751. (SCI, *corresponding author) 
12. Chao HM, Huang HX, Chang PH, Tseng KC, Miyajima A, Chern E*. Y-box binding protein-1 promotes hepatocellular carcinoma-initiating cell progression and tumorigenesis via Wnt/β-catenin pathway. Oncotarget. 2017;10;8(2):2604-2616 (SCI, *corresponding author) 
13. Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY (Chern E), Tanaka M, Miyajima A. CPM is a useful cell surface marker to isolate expandable bi-potential liver progenitor cells derived from human iPS cells. Stem Cell Reports. 2015; 5: 1-8 (SCI) 
14. Hirose Y, Saijou E, Sugano Y, Takeshita F, Nishimura S, Nonaka H, Chen YR (Chern E), Sekine K, Kido T, Nakamura T, Kato S, Kanke T, Nakamura K, Nagai R, Ochiya T, Miyajima A. Inhibition of Stabilin-2 elevates circulating hyaluronic acid levels and prevents tumor metastasis. Proc Natl Acad Sci U S A. 2012; 109(11): 4263-8. (SCI) 
15. Chen YR (Chern E), Sekine K, Nakamura K, Yanai H, Tanaka M, Miyajima A. Y-box binding protein-1 down-regulates expression of carbamoyl phosphate synthetase-I by suppressing CCAAT enhancer-binding protein-alpha function in mice. Gastroenterology. 2009; 137(1): 330-40. (SCI) 
16. Sekine K, Chen YR (Chern E), Kojima N, Ogata K, Fukamizu A, Miyajima A. Foxo1 links insulin signaling to C/EBPα and regulates gluconeogenesis during liver development. The EMBO journal. 2007; 26(15): 3607-15. (SCI) 
17. Huang CL, Chen YR (Chern E), Liu WH. Production of androstenones from phytosterol by mutants of Mycobacterium sp. Enzyme and microbial technology. 2006; 39(2): 296-300. (SCI) 
18. Chen YR (Chern E), Huang HH, Cheng YF, Tang TY, Liu WH. Expression of a cholesterol oxidase gene from Arthrobacter simplex in Escherichia coli and Pichia pastoris. Enzyme and microbial technology. 2006; 39(4): 854-60. (SCI)