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Associate Professor Feng-Ting Huang
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Associate Professor Feng-Ting Huang

Feng-Ting Huang

Title Associate Professor 
Education Ph.D., Dept. of Biochemistry and Molecular Biology, University of Southern California
Research Expertise Molecular Biology, Biochemistry
LAB Molecular Biology Lab. (AC2-414)
TEL +886-2-3366-4083
E-mail fthuang@ntu.edu.tw
Personal webpage  

Research


  • The research interest of my laboratory is focusing on class switch recombination (CSR). To effectively defend against various infectious and foreign substances in the environment, vertebrates have evolved complex immune systems. The adaptive immune system is essential for generation of immunoglobulins specifically and tight binding to these foreign substances. Various isotypes of immunoglobulins have different effector functions for optimal immune responses to pathogens. CSR is responsible for changing the heavy chain isotype from IgM to IgA, IgE or IgG.  Unlike other majority of physiological recombination systems are site-specific, CSR is rather a regionally-specific DNA recombination process. The recombination occurs at two switch regions, one is the donor Sm, and the other is one of the acceptor switch regions, including Sa, Se, and Sg regions.  In mammals, switch regions have unusual sequence features which a different mechanism for CSR from other recombination systems. Switch regions are GC-rich, especially G-rich on the non-template strand DNA.  In addition, they consist of 25-80 bp of repetitive sequence with various length of 1 to 10 kb long. All switch regions have promoters that respond to antigen stimulation, and the transcription with its product, sterile transcript, are required for CSR.  Until now, the detailed molecular mechanism of CSR is not fully comprehended. For studying the molecular mechanisms of CSR, we used microarray and proteomic approaches to screen potential important genes in the pathway.  With the gene knockdown or gene knockout experiments, the importance of these candidate genes in CSR will be investigated.  
  • The other research focus in my laboratory is on screening or identifying the molecular biomarkers of pathologic diseases by molecular biotechnologies, such as phage display library. With those novel targeting subjects been discovered, targeting ligands can be designed to advance the new field of translational molecular imaging and the potential drug delivery strategy. Imaging agents to image fundamental cellular events in living subjects can be developed and tracked with multiple non-invasive imaging systems, such as optical, MR and radiolabeled probes which are under active investigation. These imaging approaches are expected to have a fundamental impact in the study of cancer biology, as well as in molecular therapeutics including radiation therapy or chemo-therapy.

Publications


  1. Lin JJ#, Chuang CP#, Lin JY, Huang FT*, Huang CW*. Rational design, pharmacomodulation, and synthesis of [68Ga]Ga-Alb-FAPtp-01, a selective tumor-associated fibroblast activation protein tracer for PET imaging of glioma. ACS Sensors. 2021; Aug 20. (#equal contribution; SCI, *corresponding author)
  2. Huang CW, Chuang CP, Chen YJ, Wang HY, Lin JJ, Huang CY, Wei KC, Huang FT*. Integrin α2β1-targeting ferritin nanocarrier traverses the blood-brain barrier for effective glioma chemotherapy. J Nanobiotechnology. 2021;19(1):180. (SCI, *corresponding author)
  3. Huang CW, Chang YH, Lee HH, Wu JY, Huang JX, Chung YH, Hsu ST, Chow LP, Wei KC, Huang FT*. Irisin, an exercise myokine, potently suppresses tumor proliferation, invasion, and growth in glioma. FASEB J 2020; 34(7): 9678-93. (SCI,*corresponding author)
  4. Huang CW, Hsieh WC, Hsu ST, Lin YW, Chung YH, Chang WC, Chiu H, Lin YH, Wu CP, Yen TC*, Huang FT*. The use of PET imaging for prognostic integrin α2β1 phenotyping to detect non-small cell lung cancer and monitor drug resistance responses. Theranostics. 2017; 7(16):4013-28. (SCI,*corresponding author)
  5. Zheng S, Vuong BQ, Vaidyanathan B, Lin JY, Huang FT, Chaudhuri J. Non-coding RNA generated following Lariat debranching mediates targeting of AID to DNA. Cell. 2015; 161(4): 762-73.(SCI)
  6. Chung YH, Hsu PH, Huang CW, Hsieh WC, Huang FT, Chang WC, Chiu H, Hsu ST, Yen TC. Evaluation of prognostic integrin alpha2beta1 PET tracer and concurrent targeting delivery using focused ultrasound for brain glioma detection. Mol Pharm 2014; 11(11): 3904-14.(SCI)
  7. Kao YP, Hsieh WC, Hung ST, Huang CW, Lieber MR, Huang FT*. Detection and characterization of R-loops at the murine immunoglobulin Sα region. Mol Immunol. 2013; 54(2): 208-16. (SCI, *corresponding author)
  8. Huang FT, Yu K, Balter BB, Selsing E, Oruc Z, Khamlichi AA, Hsieh CL, Lieber MR. Sequence dependence of chromosomal R-loops at the immunoglobulin heavy-chain Smu class switch region. Mol Cell Biol. 2007; 27(16): 5921-32. (SCI)
  9. Huang FT, Yu K, Hsieh CL, Lieber MR. Downstream boundary of chromosomal R-loops at murine switch regions: implications for the mechanism of class switch recombination. Proc Natl Acad Sci U S A. 2006; 103(13): 5030-5. (SCI)
  10. Yu K, Roy D, Huang FT, Lieber MR. Detection and structural analysis of R-loops. Methods Enzymol. 2006; 409: 316-29. (SCI)
  11. Yu K#, Huang FT#, Lieber MR. DNA substrate length and surrounding sequence affect the activation-induced deaminase activity at cytidine. J Biol Chem. 2004; 279(8): 6496-500. (SCI, #equal contribution) 

  • 上版日期：2023-03-12

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Department of Biochemical Science & Technology, National Taiwan University,

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Last Updated

2024-11-22