NTUBST2023EN

Heterologous Gene Expression in Methylotrophic Yeasts

1. Enhancement of recombinant protein production in methylotrophic yeasts by glycerol starvation.
2. Development of a non-drug resistant and high throughput screening system in methylotrophic yeasts using flow cytometry.
3. Fermentation processes of methylotropic yeasts.

Mushroom Molecular Pharming

1. Development of gene transformation system for edible mushrooms
2. Enhancement of the heterologous gene expression in transgenic mushrooms by molecular strategy
3. Development of heterologous gene selection in edible mushrooms by single point mutaion of homologous succinate dehydrogenase
4. Development of edible vaccines in mushrooms.

1. Chen YL, Huang CT. Establishment of a two-step purification scheme for tag-free recombinant Taiwan native norovirus P and VP1 proteins.  J Chromatogr B Analyt Technol Biomed Life Sci. 2020;1159: 122357. (SCI)
2. Huang LH, Lin HY, Lyu YT, Gung GL, Huang, CT. Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B. Food Technol Biotechnol 2019; 57: 105-112. (SCI)
3. CH Chang, HA Hsiung, KL Hong, CT Huang. Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1. BMC Biotechnol 2018; 18(1): 81.(SCI)
4. YL Chen, PJ Chang, CT Huang. Small P particles formed by the Taiwan-native norovirus P domain overexpressed in Komagataella pastoris. Appl Microbiol Biotechnol 2018; 102(22): 9707-18.(SCI)
5. Lin YJ, Liu WT, Stark H, Huang CT. Expression of enterovirus 71 virus-like particles in transgenic enoki (Flammulina velutipes). Appl Microbiol Biotechnol 2015; 99(16): 6765-74.(SCI)
6. Wang HC, Chen YC, Huang CT, Hseu RS. Cloning and characterization of a thermostable and pH-stable cellobiohydrolase from Neocallimastix patriciarum J11. Protein Expr Purif2013; 90(2): 153-9.(SCI)
7. Lin YJ, Huang LH, Huang CT. Enhancement of Heterologous Gene Expression in Flammulina velutipes Using Polycistronic Vectors Containing a Viral 2A Cleavage Sequence. PLoS ONE 2013; 8(3): e59099.(SCIE)
8. Tsai T, Chien HF, Wang TH, Huang CT, Ker YB, Chen CT. Chitosan augments photodynamic inactivation of gram-positive and gram-negative bacteria. Antimicrob Agents Chemother2011; 55(5): 1883-90.(SCI)
9. Huang WT, Tai R, Hseu RS, Huang CT. Overexpression and characterization of a thermostable, pH-stable and organic solvent-tolerant Ganoderma fornicatum laccase in Pichia pastoris. Process Biochem 2011; 46(7): 1469-74.(SCI)
10. Kuo CY, Chou SY, Hseu RS, Huang CT. Heterologous expression of EGFP in enoki mushroom Flammulina velutipes. Bot Stud 2010; 51(3): 303-9.(SCI)
11. Lin YL, Liang YC, Tseng YS, Huang HY, Chou SY, Hseu RS, Huang CT, Chiang BL. An immunomodulatory protein, Ling Zhi-8, induced activation and maturation of human monocyte-derived dendritic cells by the NF-kappaB and MAPK pathways. J Leukoc Biol 2009; 86(4): 877-89.(SCI)
12. Chen CS, Huang CT, Hseu RS. Identification of Cordyceps sinensis by 18S nrDNA Sequencing and Characterization of Fermented Products in Taiwan. Food Biotechnol 2009; 23(3): 191-9.(SCIE)
13. Tsai CT, Huang CT. Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia methanolica. Enzyme Microb Technol2008; 42(6): 459-65.(SCI)
14. Kuo CY, Huang CT. A reliable transformation method and heterologous expression of beta-glucuronidase in Lentinula edodes. J Microbiol Methods 2008; 72(2): 111-5.(SCI)
15. Hsu HY, Hua KF, Wu WC, Hsu J, Weng ST, Lin TL, Liu CY, Hseu RS, Huang CT. Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells. J Cell Physiol 2008; 215(1): 15-26.(SCI)
16. Huang YH, Huang CT, Hseu RS. Effects of dockerin domains on Neocallimastix frontalisxylanases. FEMS microbiology letters 2005; 243(2): 455-60.(SCI)
17. Lin HY, Chen CT, Huang CT. Use of merocyanine 540 for photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells. Appl Environ Microbiol 2004; 70(11): 6453-8.(SCI)
18. Lee CF, Lee CJ, Chen CT, Huang CT. [delta]-Aminolaevulinic acid mediated photodynamic antimicrobial chemotherapy on Pseudomonas aeruginosa planktonic and biofilm cultures. J Photochem Photobiol B 2004; 75(1-2): 21-5.(SCI)
19. Kuo CY, Chou SY, Huang CT. Cloning of glyceraldehyde-3-phosphate dehydrogenase gene and use of the gpd promoter for transformation in Flammulina velutipes. Appl Microbiol Biotechnol 2004; 65(5): 593-9.(SCI)
20. Chen CC, Wu PH, Huang CT, Cheng KJ. A Pichia pastoris fermentation strategy for enhancing the heterologous expression of an Escherichia coli phytase. Enzyme Microb Technol 2004; 35(4): 315-20.(SCI)
21. Shih PC, Huang CT. Effects of quorum-sensing deficiency on Pseudomonas aeruginosa biofilm formation and antibiotic resistance. Journal of Antimicrobial Chemotherapy 2002; 49(2): 309-14.(SCI)
22. Chen CC, Hunag CT, Cheng KJ. Improvement of phytase thermostability by using sorghum liquor wastes supplemented with starch. Biotechnol Lett 2001; 23(5): 331-3.(SCI)
23. Huang CT, Shih PC. Effects of quorum sensing signal molecules on the hydrogen peroxide resistance against planktonic Pseudomonas aeruginosa. Journal of Microbiology Immunology and Infection 2000; 33(3): 154-8.(SCIE)
24. Huang CT, Stewart PS. Reduction of polysaccharide production in Pseudomonas aeruginosa biofilms by bismuth dimercaprol (BisBAL) treatment. Journal of Antimicrobial Chemotherapy 1999; 44(5): 601-5.(SCI)
25. Hassett DJ, Ma JF, Elkins JG, McDermott TR, Ochsner UA, West SEH, Huang CT, Fredericks J, Burnett S, Stewart PS, McFeters G, Passador L, Iglewski BH. Quorum sensing in Pseudomonas aeruginosa controls expression of catalase and superoxide dismutase genes and mediates biofilm susceptibility to hydrogen peroxide. Mol Microbiol 1999; 34(5): 1082-93.(SCI)
26. Xu KD, Stewart PS, Xia F, Huang CT, McFeters GA. Spatial physiological heterogeneity in Pseudomonas aeruginosa biofilm is determined by oxygen availability. Appl Environ Microbiol 1998; 64(10): 4035-9.(SCI)
27. Huang CT, Xu KD, McFeters GA, Stewart PS. Spatial patterns of alkaline phosphatase expression within bacterial colonies and biofilms in response to phosphate starvation.Appl Environ Microbiol 1998; 64(4): 1526-31.(SCI)
28. Stewart PS, Camper AK, Handran SD, Huang CT, Warnecke M. Spatial Distribution and Coexistence of Klebsiella pneumoniae and Pseudomonas aeruginosa in Biofilms Microbial Ecology 1997; 33(1): 2-10.(SCI)
29. Wentland EJ, Stewart PS, Huang CT, McFeters GA. Spatial variations in growth rate within Klebsiella pneumoniae colonies and biofilm. Biotechnology progress 1996; 12(3): 316-21.(SCI)
30. Huang CT, McFeters GA, Stewart PS. Evaluation of physiological staining, cryoembedding and autofluorescence quenching techniques on fouling biofilms. Biofouling 1996; 9(4): 269-77.(SCIE)
31. Huang CT, James G, Pitt WG, Stewart PS. Effects of ultrasonic treatment on the efficacy of gentamicin against established Pseudomonas aeruginosa biofilms. Colloids and Surfaces B: Biointerfaces 1996; 6(4-5): 235-42.(SCI)
32. Huang CT, Yu FP, McFeters GA, Stewart PS. Nonuniform spatial patterns of respiratory activity within biofilms during disinfection. Appl Environ Microbiol 1995; 61(6): 2252-6.(SCI)
33. Bryers JD, Huang CT. Recombinant plasmid retention and expression in bacterial biofilm cultures. Water Science and Technology 1995; 31(1): 105-15.(SCIE)
34. Huang C-T, Peretti SW, Bryers JD. Effects of inducer levels on a recombinant bacterial biofilm formation and gene expression. Biotechnol Lett 1994; 16(9): 903-8.(SCI)
35. Huang CT, Peretti SW, Bryers JD. Effects of medium carbon-to-nitrogen ratio on biofilm formation and plasmid stability. Biotechnology and bioengineering 1994; 44(3): 329-36.(SCI)
36. Huang CT, Peretti SW, Bryers JD. Plasmid retention and gene expression in suspended and biofilm cultures of recombinant Escherichia coli DH5alpha(pMJR1750). Biotechnology and bioengineering 1993; 41(2): 211-20.(SCI)
37. Huang CT, Peretti S, Bryers J. Use of flow cell reactors to quantify biofilm formation kinetics. Biotechnology Techniques 1992; 6(3): 193-8.(SCI)